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Lonicereae flos Aqua-Acupuncture Solution (LFAS) and Lonicerae flos Water-Extracted solution (LFWS) was prepared and tested for their potential antitumor activities. It was shown to possess considerable toxicity toward various tumor cell lines. Concentration of LFAS at 10 × and 5 × resulted in more than 50% inhibition of growth in ehrlich ascites tumor cells(EATC), Hepalclc7, and HeLa cells After 72 hours. Toxicity of LFWS to A549 revealed that more than 50% inhibition of growth at LFAS concentration of 10× after 72 hours concentration of LFWS at 10× and 5× showed more than 50% inhibition of grwth with EATC, Hepalclc7, HeLa and A549. In morphological study, the number of cells was decreased, and the shape of cells was round form in EATC, Hepalclc7, HeLa and A549. The organ-toxicity of LFAS to male ICR mice was studied by the measurements of GOT, GPT, LDH, and ALP-s activities after injection of LFAS for 7days. The activities of GOT, LDH and ALP-s were decreased with the LFAS. The activity of GPT was not changed with the LFAS.
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